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Purpose: - To prepare a standard solution of know concentration of copper(II)nitrate trihydrate. To prepare various dilutions of the standard solution. To measure the % transmittance or absorbance of the solutions using the spectrophotometer and to construct a calibration graph from the data. To obtain the concentration of an unknown solution using the calibration graph.
Materials: - 100ml beaker, spectrophotometer, safety goggles, test-tube rack, 5 test tube.
Method: - 1. Obtained 5 test tubes (18mm x 150mm) and labeled them from A to E.
2. In test tube A, placed about 10ml of your stock 0.500ml Copper(II) Nitrate Trihydrate solution.
3. A diluted solution was prepared by placing 8.0ml of the stock in a 25ml graduated cylinder and added distilled water until the solution was up to the 20.0ml mark
4. Step 3 was repeated using 10.0ml of solution B and 100 ml of distilled of water. This diluted was transferred to test tube C.
5. Step 3 was repeated using 10.0ml of solution C and 100 ml of distilled of water. This diluted was transferred to test tube D.
6. Step 3 was repeated using 10.0ml of solution D and 100 ml of distilled of water. This diluted was transferred to test tube E.
7. The concentration of the diluted solution B to E was calculated.
8. Solution A to E was transferred into five curvettes or five 13mm x 100 mm test tubes.
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Spectroscopy, Photochemistry, Spectrophotometry, Analytical chemistry, Bradford protein assay
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